normal human keratinocytes nhek cell lines  (PromoCell)

Journal: International journal of biological macromolecules

Article Title: Design and development of multibiocomponent hybrid alginate hydrogels and lipid nanodispersion as new materials for medical and cosmetic applications.

doi: 10.1016/j.ijbiomac.2024.134405

Figure Lengend Snippet: Fig. 22. Short-term cytotoxicity after 1 h (A) and 3 h (B) incubation with tested materials on NHEK cells.

Article Snippet: Normal human dermal fibroblast (NHDF) and normal human keratinocytes (NHEK) cell lines were purchased from PromoCell.

Techniques: Incubation

Journal: International journal of biological macromolecules

Article Title: Design and development of multibiocomponent hybrid alginate hydrogels and lipid nanodispersion as new materials for medical and cosmetic applications.

doi: 10.1016/j.ijbiomac.2024.134405

Figure Lengend Snippet: Fig. 23. Long-term cytotoxicity on NHDF (A) and NHEK (B) cells. Cells were pre-incubated with tested materials for 1 h and 3 h.

Article Snippet: Normal human dermal fibroblast (NHDF) and normal human keratinocytes (NHEK) cell lines were purchased from PromoCell.

Techniques: Incubation

Journal: Journal of Inflammation Research

Article Title: The Effect of Osthole on Transient Receptor Potential Channels: A Possible Alternative Therapy for Atopic Dermatitis

doi: 10.2147/JIR.S425978

Figure Lengend Snippet: The level of IL-4 ( A and B ) and IL-13 ( C and D ) after incubation with histamine (Hist; 100 µg/mL), lipopolysaccharides (LPS; 2 µg/mL) alone and in mixtures with osthole (OST; 0.125 and 0.5 mg/mL) and clobetasol propionate (CP; 0.5 mg/mL) in Normal Human Epithelial Keratinocytes (NHEK; ( A and C )) and prepared model of 3D skin (3D skin; ( B and D )). The horizontal line shows the mean and the bars show the standard deviation. Statistically significant differences (Two-way ANOVA with Tukey’s multiple comparisons test) compared to control (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) and to cells treated with histamine or LPS ( # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001) are marked.

Article Snippet: In brief, the Normal Human Epidermal Keratinocyte (NHEK) cell line was purchased from PromoCell GmbH (Heidelberg, Germany, cat. no. C-12005) and cultured in T-75 flasks in keratinocyte medium (Keratinocyte Growth Medium 2 ready to use, PromoCell, Heidelberg, Germany, cat. No. C-20011).

Techniques: Incubation, Standard Deviation, Control

Journal: Journal of Inflammation Research

Article Title: The Effect of Osthole on Transient Receptor Potential Channels: A Possible Alternative Therapy for Atopic Dermatitis

doi: 10.2147/JIR.S425978

Figure Lengend Snippet: Histamine receptor 4 ( A and B ) and IL-4 receptor-α ( C and D ) gene expression level after incubation with histamine (Hist; 100 µg/mL), lipopolysaccharides (LPS; 2 µg/mL) alone and in mixtures with osthole (OST; 0.125 and 0.5 mg/mL) and clobetasol propionate (CP; 0.5 mg/mL) in Normal Human Epithelial Keratinocytes (NHEK; ( A and C )) and prepared model of 3D skin (3D skin; ( B and D )). The horizontal line shows the mean and the bars show the standard deviation. Statistically significant differences (Two-way ANOVA with Tukey’s multiple comparisons test) compared to control (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) and to cells treated with histamine or LPS (# p < 0.05, ### p < 0.001, #### p < 0.0001) are marked.

Article Snippet: In brief, the Normal Human Epidermal Keratinocyte (NHEK) cell line was purchased from PromoCell GmbH (Heidelberg, Germany, cat. no. C-12005) and cultured in T-75 flasks in keratinocyte medium (Keratinocyte Growth Medium 2 ready to use, PromoCell, Heidelberg, Germany, cat. No. C-20011).

Techniques: Gene Expression, Incubation, Standard Deviation, Control

Journal: Journal of Inflammation Research

Article Title: The Effect of Osthole on Transient Receptor Potential Channels: A Possible Alternative Therapy for Atopic Dermatitis

doi: 10.2147/JIR.S425978

Figure Lengend Snippet: TRPV1 ( A and B ), TRPV4 ( C and D ) and TRPM8 ( E and F ) gene expression level after incubation with histamine (Hist; 100 µg/mL), lipopolysaccharides (LPS; 2 µg/mL) alone and in mixtures with osthole (OST; 0.125 and 0.5 mg/mL) and clobetasol propionate (CP; 0.5 mg/mL) in Normal Human Epithelial Keratinocytes (NHEK; ( A, C and E )) and prepared model of 3D skin (3D skin; ( B, D and F )). The horizontal line shows the mean and the bars show the standard deviation. Statistically significant differences (Two-way ANOVA with Tukey’s multiple comparisons test) compared to control (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) and to cells treated with histamine or LPS ( # p < 0.05, ### p < 0.001, #### p < 0.0001) are marked.

Article Snippet: In brief, the Normal Human Epidermal Keratinocyte (NHEK) cell line was purchased from PromoCell GmbH (Heidelberg, Germany, cat. no. C-12005) and cultured in T-75 flasks in keratinocyte medium (Keratinocyte Growth Medium 2 ready to use, PromoCell, Heidelberg, Germany, cat. No. C-20011).

Techniques: Gene Expression, Incubation, Standard Deviation, Control

Journal: Aging (Albany NY)

Article Title: Curcumol may alleviate psoriasis-like inflammation by inhibiting keratinocyte proliferation and inflammatory gene expression via JAK1/STAT3 signaling

doi: 10.18632/aging.203287

Figure Lengend Snippet: Curcumol inhibits proliferation and cell cycle progression and promote apoptosis in stimulated human keratinocytes. ( A ) CCK-8 cell viability assay in NHEK cells treated with curcumol (0, 5, 10, 20, 40 or 80 μM) for 24 h. ( B ) CCK-8 cell viability assay in NHEK cells pre-incubated with curcumol (10 or 20 μM) for 24 h, and stimulated with 10 ng/mL of M5 mix for 24 h. ( C ) Relative fluorescence levels were quantified by EdU and DAPI staining. ( D , E ) Flow cytometer assay was used to analyze cell apoptosis. ( F ) Cell cycle distribution was measured by flow cytometry. ( G – I ) Western blot was performed to measure the expressions of p27 Kip1 and CDK4 in NHEK cells. **P<0.01 compared with control group; # P<0.05, ## P<0.01 compared with 10 ng/mL_M5 group.

Article Snippet: Normal human epidermal keratinocytes (NHEK) cell line was purchased from ScienCell Research Laboratories (Carlsbad, CA, USA).

Techniques: CCK-8 Assay, Viability Assay, Incubation, Fluorescence, Staining, Flow Cytometry, Western Blot, Control

Journal: Aging (Albany NY)

Article Title: Curcumol may alleviate psoriasis-like inflammation by inhibiting keratinocyte proliferation and inflammatory gene expression via JAK1/STAT3 signaling

doi: 10.18632/aging.203287

Figure Lengend Snippet: Curcumol suppresses inflammatory gene expression in M5-treated NHEK cells. NHEK cells were preincubated with curcumol (10 or 20 μM) for 24 h, stimulated with M5 (10 ng/mL) for 24 h, and gene expression of IL-6 ( A ), IL-1β ( B ), CXCL1 ( C ), CXCL2 ( D ), LL37 ( E ), β-defensin-2 ( F ), S100A7 ( G ), and S100A8 ( H ) was analyzed by RT-qPCR. **P<0.01 compared with control group; # P<0.05, ## P<0.01 compared with 10 ng/mL_M5 group.

Article Snippet: Normal human epidermal keratinocytes (NHEK) cell line was purchased from ScienCell Research Laboratories (Carlsbad, CA, USA).

Techniques: Gene Expression, Quantitative RT-PCR, Control

Journal: Aging (Albany NY)

Article Title: Curcumol may alleviate psoriasis-like inflammation by inhibiting keratinocyte proliferation and inflammatory gene expression via JAK1/STAT3 signaling

doi: 10.18632/aging.203287

Figure Lengend Snippet: Curcumol attenuates M5-induced oxidative stress damage in NHEK cells. NHEK cells were treated with curcumol (10 or 20 μM) for 24 h, and then stimulated with M5 (10 ng/mL) for 24 h. ( A ) ROS production in NHEK cells was detected by flow cytometry. ( B – E ) ELISA was used to detect the levels of SOD ( B ), MDA ( C ), GSH ( D ), and CAT ( E ) in the supernatants of NHEK cells. **P<0.01 compared with control group; # P<0.05, ## P<0.01 compared with 10 ng/mL_M5 group.

Article Snippet: Normal human epidermal keratinocytes (NHEK) cell line was purchased from ScienCell Research Laboratories (Carlsbad, CA, USA).

Techniques: Flow Cytometry, Enzyme-linked Immunosorbent Assay, Control

Journal: Aging (Albany NY)

Article Title: Curcumol may alleviate psoriasis-like inflammation by inhibiting keratinocyte proliferation and inflammatory gene expression via JAK1/STAT3 signaling

doi: 10.18632/aging.203287

Figure Lengend Snippet: Curcumol promotes differentiation in M5-stimulated NHEK cells. NHEK cells were treated with curcumol (10 or 20 μM) for 24 h, and then stimulated with M5 (10 ng/mL) for 24 h. ( A ) Expression of keratin 1, keratin 5, keratin 10, filaggrin and loricrin in NHEK cells was analyzed by western blotting. ( B – F ) The relative expression of keratin 1 ( B ), keratin 5 ( C ), keratin 10 ( D ), filaggrin ( E ), and loricrin ( F ) quantified via normalization to β-actin. **P<0.01 compared with control group; # P<0.05, ## P<0.01 compared with 10 ng/mL_M5 group.

Article Snippet: Normal human epidermal keratinocytes (NHEK) cell line was purchased from ScienCell Research Laboratories (Carlsbad, CA, USA).

Techniques: Expressing, Western Blot, Control

Journal: Aging (Albany NY)

Article Title: Curcumol may alleviate psoriasis-like inflammation by inhibiting keratinocyte proliferation and inflammatory gene expression via JAK1/STAT3 signaling

doi: 10.18632/aging.203287

Figure Lengend Snippet: Curcumol inhibits proliferation of M5-treated NHEK cells via downregulation of p-STAT3/cyclin D2 axis. NHEK cells were treated with curcumol (10 or 20 μM) for 24 h, and then stimulated with M5 (10 ng/mL) for 24 h. ( A ) The expression level of p-STAT3 in NHEK cells was detected by western blotting, and normalized to STAT3. ( B ) The nuclear levels of p-STAT3 were analyzed by western blotting, and normalized to Histone H3. ( C ) Cyclin D2 mRNA levels were analyzed by RT-qPCR. ( D ) Cyclin D2 protein levels were analyzed by western blotting, and normalized to β-actin. **P<0.01 compared with control group; ## P<0.01 compared with 10 ng/mL_M5 group.

Article Snippet: Normal human epidermal keratinocytes (NHEK) cell line was purchased from ScienCell Research Laboratories (Carlsbad, CA, USA).

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Control

Journal: Aging (Albany NY)

Article Title: Curcumol may alleviate psoriasis-like inflammation by inhibiting keratinocyte proliferation and inflammatory gene expression via JAK1/STAT3 signaling

doi: 10.18632/aging.203287

Figure Lengend Snippet: Curcumol inhibits proliferation of M5-treated NHEK cells via downregulation of JAK1/STAT3 signaling. ( A ) NHEK cells were treated with curcumol (10 or 20 μM; 24 h), and stimulated with M5 (10 ng/mL; 24 h). Levels of p-JAK1 were analyzed by western blotting, and normalized to JAK1. ( B ) NHEK cells were treated with 20 μM curcumol or/and 3μM ruxolitinib (RUX) for 24 h, and then stimulated with M5 (10 ng/mL) for 24 h. Levels of p-STAT3 were analyzed by western blotting, and normalized to STAT3. **P<0.01 compared with control group; # P<0.05, ## P<0.01 compared with M5 group; ^^P<0.01 compared with M5 + RUX group.

Article Snippet: Normal human epidermal keratinocytes (NHEK) cell line was purchased from ScienCell Research Laboratories (Carlsbad, CA, USA).

Techniques: Western Blot, Control